GENDX NGSgo-ProntoAmp for fast HLA typing with whole-gene amplification for Nanopore sequencing. Process up to 96 samples, with DNA-to-results in under 8 hours.
Enhance HLA typing with GENDX NGS-Pronto, designed for Oxford Nanopore Technologies. Amplify 11 loci with full-gene coverage, complete barcoded library prep, and rapid results in under 8 hours. Achieve high-resolution sequencing with a short, room-temperature workflow and seamless NGSengine-Turbo analysis.
Human Leukocyte Antigen (HLA) typing to match patients and donors is an essential step taken to promote engraftment and reduce the risk of graft- versus host-disease.
Benefits
- Amplification & barcoded library preparation
- DNA to results < 8h
- Up to 96 samples per run
Specifications:
- 4-96 samples per run
- DNA to results in < 8 hours for 12 samples
- 11 loci
- No fragmentation, full phasing
- High gene coverage
- Short and simple workflow
- All incubations at room temperature
- Long-read sequencing
- Dedicated software NGSengine Turbo
- Low equipment costs
Description
Get ready for the future of HLA typing with NGS-Pronto, our solution designed for Oxford Nanopore Technologies sequencing devices.
NGSgo-ProntoAmp offers amplification of 11 HLA loci using a whole-gene approach, resulting in at least a 3-field resolution. NGS-ProntoPrep offers a complete barcoded library preparation for sequencing on ONT devices. After nanopore sequencing, the data can be directly analysed with NGSengine®-Turbo.
NGSgo-ProntoAmp and NGS-ProntoPrep are available as RUO.
The NGSgo-ProntoAmp amplification is also compatible with the NGSgo Library Full Kit for sequencing on Illumina systems!
Amplification & Library Preparation
Covering 11 HLA loci using a comprehensive whole-gene approach, the fast thermal cycling program is completed in just 90 minutes. Library preparation is equally efficient, performed entirely at room temperature, and easily paused as needed. Additionally, there is no fragmentation – our process yields long, intact amplicons to further achieve full phasing. Depending on the sample number, library prep takes just 1.5 to 2 hours.
Compatibility
Compatible with R10.4.1 flow cells, NGS-Pronto can be sequenced on MinION Mk1B and GridION sequencers from ONT, delivering rapid results in as little as 15 minutes per sample. The assay is to be used with compatible consumables from ONT, including EXP-NBA114 and EXP-AUX003.
NGS-Pronto has been validated to be compatible with MinKNOW versions 23.04.3, 23.11.7, and 24.06.5.
Data analysis
Streamline your analysis with NGSengine-Turbo, our user-friendly software tailored for ONT data. With a fast analysis time of approximately 5 minutes per sample, you’ll quickly have the results you need.