Tecan IBL International offers a comprehensive range of ELISAs, antibodies, immunoassays and recombinant proteins for research use only.
The High Sensitivity ELISA for Interleukin-10 (IL-10) is designed to accurately measure this cytokine in low concentrations from serum, plasma, or cell culture samples. The kit's enhanced sensitivity allows for precise quantification of IL-10, even at levels below those detectable by conventional ELISAs. This increased sensitivity is achieved through signal amplification technologies integrated into the assay design.
Key Characteristics and Functions of IL-10:
- Molecular Structure: IL-10 is a member of the four α-helix bundle cytokine family and functions as a homodimer with an apparent molecular mass of 39 kDa. Despite containing an N-linked glycosylation site, it has minimal detectable carbohydrates. The recombinant IL-10 expressed in E. coli retains all known biological activities of the native cytokine.
- Production: IL-10 is produced by activated CD8(+) T cells, T-helper CD4(+) T cell clones, and other immune cells including macrophages, mast cells, and thymocytes.
- Biological Activities:
- Immune Regulation: IL-10 acts as a potent suppressor of macrophage, T-cell, and NK cell effector functions. It inhibits cytokine synthesis and several accessory cell functions of macrophages.
- Cell Function Modulation: IL-10 regulates the proliferation and differentiation of B-cells, mast cells, and thymocytes, contributing to its broad range of effects on immune cell function.
- Anti-inflammatory Effects: The cytokine is involved in downregulating inflammatory responses, making it a key player in controlling chronic inflammation and autoimmune diseases.
- Genetic Information: The IL-10 gene is located on chromosome 1 and is present as a single copy in the human genome. It shows strong sequence homology with murine IL-10 and an open reading frame in the Epstein-Barr virus (BCRF1), which shares many biological activities with human IL-10 and may influence host-virus interactions.
- Clinical Implications:
- Graft Rejection: The immunosuppressive properties of IL-10 suggest its potential use in suppressing organ transplant rejections.
- Anti-inflammatory Therapy: IL-10's ability to exert strong anti-inflammatory effects makes it a candidate for therapeutic interventions in various inflammatory and autoimmune conditions.
In summary, IL-10 is a crucial cytokine with significant regulatory roles in immune responses and inflammation. The High Sensitivity ELISA for IL-10 provides valuable insights into its levels in various physiological and pathological conditions, offering potential applications in research and clinical practice related to immune regulation and inflammatory diseases.
This ELISA Kit from Tecan IBL International is for research use only.
Tecan IBL International offers a comprehensive range of ELISAs, immunoassays, antibodies and recombinant proteins.
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