Interferon-gamma (IFN-gamma) High Sensitivity ELISA

The High Sensitivity ELISA for Interferon-gamma (IFN-γ) is designed for the precise measurement of this cytokine in very low concentrations from various biological samples, including serum, plasma, or cell culture supernatants. The advanced design of the ELISA kit allows for accurate detection and quantification below conventional ELISA limits through signal amplification technology.

Key Characteristics and Functions of IFN-γ:

• Molecular Structure: IFN-γ is a homodimeric glycoprotein with two subunits, each approximately 21 to 24 kDa. It is composed of 166 amino acids, including a 20-amino-acid signal peptide. The protein exists primarily as a dimer in its biologically active form.
• Gene and Production:
• Gene Location: The IFN-γ gene is located on chromosome 12 and consists of three introns and four exons.
• Cellular Sources: Unlike IFN-alpha and IFN-beta, which can be produced by almost any cell type in response to viral infection, IFN-γ is specifically produced by T cells and natural killer (NK) cells.
• Biological Activities:
• Antiviral and Antiparasitic: IFN-γ has potent antiviral and antiparasitic effects, helping to protect the host from a range of pathogens.
• Inhibition of Cell Proliferation: It inhibits the proliferation of both normal and transformed cells, which is important in controlling cancer growth and in modulating immune responses.
• Synergistic Effects: IFN-γ works synergistically with Tumor Necrosis Factor-alpha (TNF-α) and TNF-beta to enhance its inhibitory effects on cell proliferation. This synergy can amplify the immune response and affect various cellular processes.
• Clinical Relevance:
• Immune Response: IFN-γ is crucial in the regulation and activation of the immune system. It is involved in Th1 cell differentiation and the enhancement of antigen presentation.
• Disease Association: Abnormal levels of IFN-γ are associated with various diseases, including chronic infections, autoimmune disorders, and certain cancers. Elevated levels may indicate ongoing immune activation or inflammation, while low levels might suggest impaired immune function.
• Applications:
• Research: The High Sensitivity ELISA for IFN-γ is useful for studying immune responses, cytokine interactions, and the role of IFN-γ in disease processes.
• Clinical: Monitoring IFN-γ levels can provide valuable insights into immune system function, disease progression, and therapeutic efficacy in conditions such as infections, autoimmune diseases, and cancer.

Overall, this High Sensitivity ELISA for IFN-γ is a powerful tool for detailed analysis of immune responses and cytokine dynamics, offering insights into both basic research and clinical applications.

This ELISA Kit from Tecan IBL International is for research use only.

Tecan IBL International offers a comprehensive range of ELISAs, immunoassays, antibodies and recombinant proteins.
Explore the full range >