Quantabio qScript XLT cDNA SuperMix for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR
Quantabio qScript XLT cDNA SuperMix for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR
- Stabilized 5X concentrated master mix simplifies reaction assembly and improves accuracy by minimizing pipetting steps
- Optimized, double pre-primed SuperMix provides equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
- Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure
- Perform more qPCR assays from limiting amounts of RNA with 2-3 fold improved cDNA yields over older enzyme technologies
- Broad linear dynamic detection range spanning up to 108 fold
qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant.