High transfection efficiencies in difficult-to-transfect cell lines, e.g. HeLa, IMR-90, NIH/3T3, NK-92, or Sf9, in the Nucleofector 2b Device
The Cell Line Nucleofector Kit R is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. High transfection efficiencies in difficult-to-transfect cell lines
Applications
- Transfection of cell numbers in the range of 2 × 105to 2 × 107 cells
- Low-throughput transfection in single cuvette format
This kit has been determined to be optimal for transfecting the following cell types:
- B16-F0
- BJ
- COS-7
- HeLa
- HT-29
- Hut-78
- IMR-90
- KG-1
- L6
- LNCaP
- MDBK
- NIH/3T3
- NK-92
- PANC-1
- Sf9
- WI-38
Includes:
- 25 mL Nucleofector Solution R
- 5 mL Supplement 1
- 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
- 25 Aluminum cuvette (100 µL)
- 25 Single use pipettes