Cell Line Kit R, 4D-Nucleofector X Kit, 25 reactions, 100 µL Nucleocuvette Vessels

Code: VCA-1001

High transfection efficiencies in difficult-to-transfect cell lines, e.g. HeLa, IMR-90, NIH/3T3, NK-92, or Sf9, in the Nucleofector 2b Device

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The Cell Line Nucleofector Kit R is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. High transfection efficiencies in difficult-to-transfect cell lines

Applications

  • Transfection of cell numbers in the range of 2 × 105to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

This kit has been determined to be optimal for transfecting the following cell types:

  • B16-F0
  • BJ
  • COS-7
  • HeLa
  • HT-29
  • Hut-78
  • IMR-90
  • KG-1
  • L6
  • LNCaP
  • MDBK
  • NIH/3T3
  • NK-92
  • PANC-1
  • Sf9
  • WI-38

Includes:

  • 25 mL Nucleofector Solution R
  • 5 mL Supplement 1
  • 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
  • 25 Aluminum cuvette (100 µL)
  • 25 Single use pipettes

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