Code: V4XP-3024
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format)
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The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.
- High transfection efficiencies in primary cells
- Cells maintain functionality post transfection
- Conditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector System
- Transfection of cell numbers in the range of 2 × 105to 2 × 107 cells
- Low throughput applications
This kit has been determined to be optimal for transfecting the following cell types:
- B cell (human)
- CD34+ cell (human)
- Chondrocyte (human)
- DC (human)
- DC (mouse), mature
- ES (human), H9
- ES (mouse)
- Hepatocyte (human)
- HMEC (human)
- Keratinocyte (human)
- Macrophage (human)
- Monocyte (human)
- Neuron (rat), brain Hi/Cx
- NHBE (human)
- RPE (human)
- T cell (human), stim.
- T cell (human), unstim.
- T cell (mouse), unstim.
Includes:
- 25 mL P3 Primary Cell Nucleofector Solution
- 5 mL Supplement 1
- 50 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)
- 24 x 100 µL Nucleocuvette Vessel
- 24 x Single use pipettes