For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-NucleofectorTM X Unit (20 µL format)
The P2 Primary Cell 4D-Nucleofector X Kit S is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector System
This kit has been determined to be optimal for transfecting the following cell types:
- Macrophage (mouse)
- NHDF (human), neonatal
Applications
- Transfection of low cell numbers in the range of 5 x 104to 1 x 106 cells
- Medium throughput applications
- Optimization of transfection conditions e.g. substrate titrations
Includes:
- 1 x 0.675 mL P2 Primary Cell Nucleofector Solution
- 1 x 0.15 mL Supplement 1
- 50 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)
- 2 x 16-well Nucleocuvette Strips (20 μl)