Code: V4XC-2012
For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-NucleofectorTM X Unit (100 µL format)
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High transfection efficiencies in difficult-to-transfect cell lines. The SF Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.
Applications
- Transfection of cell numbers in the range of 2 × 105to 2 × 107 cells
- Low throughput Applications
Includes:
- 2 x 0.675 mL SF Cell Line NucleofectorTM Solution
- 2 x 0.15 mL Supplement 1
- 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
- 12 x 100 µL NucleocuvetteTM Vessel
- 12 x Single use pipettes
This kit has been determined to be optimal for transfecting following cell types:
- 293-F (FreeStyle), 32D
- A-20, A2058, A375, A431, A549, AGS, ARPE-19
- BetaTC/6, BxPC-3
- C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
- EL4
- FDC-P1
- H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
- IMR-32
- 1
- K-562
- KG-1
- L-428, LNCaP
- MDA-MB-453, MDBK, Molt-4, MV-4-11
- Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
- P19, P815, PC-12, PC-3
- Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
- S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
- T2, T84, TF-1
- U266B1, U-937
- Vero