Code: V4XC-1032
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-NucleofectorTM X Unit (20 µL format)
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High transfection efficiencies in difficult-to-transfect cell lines. The SE Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.
Applications
- Transfection of low cell numbers in the range of 5 × 104to 1 × 106 cells
- Medium throughput applications
- Optimization of transfection conditions e.g. substrate titrations
Includes:
- 1 x 0.675 mL SE Cell Line Nucleofector Solution
- 1 x 0.15 mL Supplement 1
- 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0),
- 2 x 16-well Nucleocuvette Strips (20 μL)
This kit has been determined to be optimal for transfecting following cell types:
- 3T3-L1, diff., 3T3-L1, undiff.
- A-10, A7r5
- BHK-21, BJ
- C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
- DU145
- GH3
- HCT116, HeLa, HeLa S3
- IMR-90
- Jurkat (DSMZ), Jurkat E6.1
- KG-1a, L6
- MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
- NCTC clone 929
- Panc-1
- T/G-HA VSMC, T-47D, T84
- U2OS, U-87 MG
- WI-38