HMGB1 15N labelled for Mass Spectrometry - 5 µg

Full-Length HMGB1 for Mass Spectrometry and NMR Studies

Product Overview: HMGB1 (High Mobility Group Box 1) is a critical nuclear protein involved in numerous cellular processes, including gene regulation, DNA repair, and chromatin organization. It is released passively by necrotic cells, retained by apoptotic cells, and actively secreted by inflammatory cells. The essential nature of HMGB1 is underscored by the early mortality observed in HMGB1 knockout mice.

Functional Forms of HMGB1:

• Chemoattractant Activity: Mediated by the full-length HMGB1 protein, which promotes cell migration.
• Cytokine Induction: Associated with specific redox forms of HMGB1, responsible for cytokine production and immune modulation.

Product Details:

• Protein: Full-Length HMGB1
• Molecular Weight: 25 kDa
• Labeling: Isotopically labeled with 15N for enhanced resolution in mass spectrometry and NMR studies.
• Source: Produced in E. coli, with the protein sequence corresponding to human HMGB1.
• Applications: Suitable for mass spectrometry and NMR spectroscopy to facilitate detailed structural and functional analyses.

Applications:

• Structural Analysis: Ideal for investigating the detailed structure and dynamics of HMGB1 using NMR spectroscopy.
• Functional Studies: Supports in-depth functional research into HMGB1’s role in DNA binding and protein interactions.
• Mass Spectrometry: Provides accurate molecular weight determination and analysis of post-translational modifications.

Product Benefits:

• High Purity: Ensures reliable results in structural and functional studies.
• Isotopic Labeling: Enhances detection and provides precise insights into the protein's structure and interactions.

This Full-Length HMGB1 protein, labeled with 15N, is a valuable tool for researchers aiming to explore the structural and functional aspects of HMGB1 in detail.

This Immunoassay from Tecan IBL International is for research use only.

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