BoxB from HMGB1, human & mouse, with tag, LPS-free - 15 µg

BoxB: A DNA-Binding Domain of HMGB1 and Its Functional Characteristics

Overview: BoxB is one of the DNA-binding domains of the HMGB1 protein, which belongs to the High Mobility Group Box (HMG Box) family. BoxB has been shown to replicate the cytokine activity of full-length HMGB1, making it a significant protein for research into HMGB1's proinflammatory roles.

Functional Characteristics:

• Cytokine Activity: Structural and functional analyses indicate that recombinant BoxB can effectively mimic the cytokine activity of full-length HMGB1. It is capable of activating macrophages, leading to the release of tumor necrosis factor (TNF) and other proinflammatory cytokines. This activity was documented in studies by Li et al., 2004 (Mol Med, 9:37-45), showcasing BoxB's role in modulating immune responses.
• Production and Purification: BoxB is produced in E. coli using an expression plasmid that encodes the mammalian sequence for BoxB, ensuring that the protein is identical to that of both human and mouse HMGB1. To facilitate bacterial expression, the recombinant protein is preceded by an additional four amino acids (MARI). The production process and its efficiency were described in Müller et al., 2001 (Biochemistry, 40:10254-61) and Bianchi et al., 1992 (EMBO J, 11:1055-63).

Significance:

• Functional Utility: The recombinant BoxB retains the functional characteristics of native HMGB1, making it a valuable tool for investigating the proinflammatory roles of HMGB1. It allows researchers to study how HMGB1 interacts with cellular components and modulates immune responses without the complexities associated with full-length HMGB1.
• Research Applications: BoxB is particularly useful in:
• Understanding Inflammatory Mechanisms: By studying BoxB, researchers can gain insights into how HMGB1 contributes to inflammation and immune system activation.
• Developing Therapeutic Interventions: Investigating BoxB’s interactions and effects may lead to new therapeutic strategies for managing diseases associated with HMGB1 activity, such as chronic inflammation and autoimmune disorders.

Summary: BoxB, as a recombinant protein, mirrors the cytokine activity of full-length HMGB1 and is produced with a sequence that matches the native human and mouse proteins. Its ability to activate macrophages and induce proinflammatory cytokine release makes it a valuable asset for studying HMGB1's role in inflammation and immune responses.

This Immunoassay from Tecan IBL International is for research use only.

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