Amyloid-beta (1-42) ELISA

Amyloid-beta (1-42) ELISA Overview

Purpose: This ELISA assay is designed for the measurement of Amyloid-beta (Aβ) peptides, specifically Aβ (1-42), which are cleaved at the N-terminal side. The assay is particularly useful for analyzing Aβ variants that play a role in Alzheimer’s disease.

Background:

• Amyloid-beta (Aβ): Aβ peptides are derived from the amyloid precursor protein (APP) through the actions of β-secretase and γ-secretase. These peptides are implicated in the formation of amyloid plaques, which are a hallmark of Alzheimer’s disease.
• Variants: The Aβ peptide can be 40 or 42 amino acids long, with Aβ (1-42) being one of the more prominent forms found in Alzheimer’s plaques.

Significance:

• Alzheimer’s Disease: The accumulation of Aβ plaques in the brain is a key pathological feature of Alzheimer’s disease. The Aβ (1-42) form is particularly important due to its propensity to aggregate and form plaques.
• Discovery: In 1995, research by Saido et al. highlighted the differential deposition of amyloid-β peptide species, including Aβ (N3pE). This variant, starting at the 3rd amino terminal residue glutamate, converts to pyroglutamate through intramolecular dehydration, a modification linked to the progression of Alzheimer's disease.

Assay Details:

• Measurement: This ELISA specifically measures Aβ (1-42) in biological samples. It is designed to quantify the peptide's levels and track its variants.
• Application: The assay is used in research settings to study amyloid plaque formation, monitor disease progression, and evaluate therapeutic interventions targeting Aβ. It can also be employed in experimental models and patient samples to investigate the role of Aβ (1-42) in Alzheimer’s disease pathology.

This assay provides valuable insights into the amyloidogenic processes underlying Alzheimer's disease and aids in understanding the impact of various Aβ species on disease development and progression.

This ELISA Kit from Tecan IBL International is for research use only.

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