ELISA Kits and Reagents for Sensitive Immunoassay Detection
ELISA (enzyme-linked immunosorbent assay) is a widely-used application for the detection of antigens in a microwell format. The assay requires the use of an antibody labeled with a tag such as peroxidase or phosphatase, and an enzymatic substrate that produces a visible signal, indicating the presence of the antigen. Colorimetric, chemiluminescent or fluorescent systems may provide qualitative or quantitative results, where signal levels are measured in a spectrophotometer, luminometer or fluorometer. In a properly optimized assay, the intensity of the signal generated is proportional to the amount of antigen present.
KPL provides a complete line of antibodies, conjugates, reagents and kits for various ELISA applications. See the Technical Guide for ELISA for an understanding of the many factors influencing ELISA performance.
Affinity Purified Antibodies and Conjugates
KPL offers a selection of more than 500 antibodies and conjugates to over twenty animal species’ immunoglobulins.
KPL’s ELISA substrates enable colorimetric and chemiluminescent detection and offer a range of performance options.
Immunoassay Support Reagents
KPL offers a line of support reagents such as coating solutions, blockers and wash solutions that minimize assay variables.
Protein Detector ELISA Kits
KPL produces a line of universal Protein Detector ELISA Kits designed to meet the needs of users of all skill levels.