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Chemiluminescent Blotting Substrates


Chemiluminescent Blotting Substrates for Peroxidase and Phosphatase Detection

KPL’s chemiluminescent substrates facilitate detection of proteins when catalyzed with the appropriate enzyme. The light signal produced by this reaction can be recorded permanently on X-ray film or by image analysis. KPL offers highly sensitive and stable liquid chemiluminescent substrates for use in either horseradish peroxidase- or alkaline phosphatase-mediated systems.

Chemiluminescent Blotting Substrates for Peroxidase DetectionKPL - LumiGLO-01
KPL’s most sensitive substrates for protein detection on blots enable increased sensitivity over colorimetric substrates with femtogram-level detection.

  • LumiGLO UltraTM Chemiluminescent Substrate produces an intense signal for hours with extremely low background. This substrate is optimized for use with image analysis systems and is ideal for detecting poorly expressed proteins.
  • LumiGLO ReserveTM Chemiluminescent Substrate is one of the most sensitive substrates available for Western blotting. This substrate is recommended for use with film and has a wide dynamic range that is linear for a long period of time.
  • LumiGLO® Chemiluminescent Substrate provides picogram-level detection and is an effective and economical alternative to more sensitive chemiluminescent substrates.

 

Chemiluminescent Blotting Substrates for Phosphatase DetectionKPL - Phospha GLO-01

  • PhosphaGLO ReserveTM Chemiluminescent Substrate is ideal for detection of proteins expressed in femtogram concentrations. It produces a sustained signal for days, enabling generation of multiple image exposures.
  • PhosphaGLOTM Chemiluminescent Substrate provides picogram detection of phosphatase-labeled conjugates and is suitable for routine blotting applications. Superior glow time allows generation of multiple exposures.

 

Cat# Product Size
 54-61-00  LumiGLO Chemiluminescent Substrate  240 mL Quote me
 54-61-01  LumiGLO Chemiluminescent Substrate  720 mL Quote me
 54-61-02  LumiGLO Chemiluminescent Substrate  60 mL Quote me
 54-71-00  LumiGLO Reserve™ Chemiluminescent Substrate  2400 cm2 Quote me
 54-71-01  LumiGLO Reserve™ Chemiluminescent Substrate  600 cm2 Quote me
 54-71-02  LumiGLO Reserve™ Chemiluminescent Substrate  1000 cm2 Quote me
 54-51-00  LumiGLO Ultra™ Western Blotting Substrate  50 mL Quote me
 55-60-03  PhosphaGLO™ AP Substrate  30 mL Quote me
 55-60-04  PhosphaGLO™ AP Substrate  100 mL Quote me
 55-60-01  PhosphaGLO™ Reserve AP Substrate  30 mL Quote me
 55-60-02  PhosphaGLO™ Reserve AP Substrate  100 mL Quote me
 54-56-00  SignaLOCK™ AP ChemiWestern Kit (Imager/Film)  1000 cm2 Quote me
 54-54-00  SignaLOCK™ HRP ChemiWestern Kit (Imager)  1000 cm2 Quote me
 54-53-00 SignaLOCK™ HRP ChemiWestern Kit (Film)  1000 cm2 Quote me