KPL – see more with KPL protein detection and analysis tools
KPL provide high quality protein detection and analysis tools that increase signal and reduce background in immunoassays, including:
- Secondary Antibodies and Conjugates
- Anti-Bacteria Primary Antibodies
- ELISA Kits and Reagents
- Western Blotting Kits and Reagents
- Immunohistochemistry Kits and Reagents
- Recombinant Protein Detection and Analysis
- Sample Preparation and Protein Purification
- Protein Labeling Kits and Reagents
- Support Reagents and Accessories
- Nucleic Acid Labeling and Detection Kits
KPL offers over 500 reagents and assay kits that provide exceptional performance and consistent quality in ELISA, blotting and immunohistochemistry applications.
KPL supplies antibodies and a full line of immunoassay reagents to in vitro diagnostic, biopharmaceutical, biotech and food pathogen test manufacturers around the world.
KPL reagents are integral components of commercially available human and animal in vitro diagnostic kits, in-process testing protocols, and high-throughput screening applications.
Protein Detector™ Western Blot Kits and substrates
KPL offers an extensive line of stable, liquid chromogenic and chemiluminescent substrates, optimised specifically for membrane-base assays.
KPL blotting substrates are designed as pre-formulated, convenient systems that are easy to use and provide a high degree of assay to assay precision, maximum stability and sensitivity.
Choosing a Western Blotting detection method
Compare and choose the optimal kit for your application:
|Format||2-Component: 1:2 mix and use||2-Component: 1:1 mix and use||1-Component ready-to-use||1-Component ready-to-use|
|Detection Limit||Sub-pictogram; femtogram||6 pictogram||50 pictogram||500 pictogram|
|Detection Method||Film or image analysis||Film or image analysis||Visual – dark blue ppt||Visual – purple ppt|
|Stablity of Working Solution||8 hours||24 hours||1 year||1 year|
|Duration Signal||4 – 8 hours||1 – 2 hours||NA||NA|
|Recommended Membrane||Nitrocellulose or PVDF||Nitrocellulose or PVDF||Nitrocellulose or PVDF||Nitrocellulose or PVDF|
Protein Detector ELISA Substrates
KPL offers a variety of ELISA substrates that are tested to assure lot-to-lot consistency, reproducibility and long-term stability.
As stable liquid systems, they provide convenience and a range of sensitivity for ELISA applications. Substrate systems are made available to react with either alkaline phosphatase or horseradish peroxidase.
KPL ELISA Substrates
Compare and choose the best visualisation method for your ELISA application:
|Substrate||pNPP||ABTS||Sureblue/TMB||Sureblue Reserve||LumiGLO||LumiGLO Reserve|
|Chromogenic||Chromogenic||Chromogenic||Chemilu- minescent||Chemilu- minescent|
|Kit components||2||1 or 2||1 or 2||1||2||2|
|Detection limit||10-13 moles of AP||10-13 moles of HRP||10-15 moles of HRP||5×10-16 moles of HRP||10-18 moles of HRP||5×10-19 moles of HRP|
|Stability of working solution||24 hours||1-C: min 1 year
2-C: 24 hours
|1-C: min 2 year
2-C: 24 hours
|2 years from DOM||24 hours||8 hours at RT|
|Absorbance before stopping||405-410nm||405-410nm||650nm||650nm||425 (emission)||425 (emission)|
|Absorbance after stopping||405-410nm||405-410nm||450nm||450nm||425 (emission)||425 (emission)|